PrimePCR™ SYBR® Green Assay: Cdo1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0009581
Assay Design:   intron_spanning
Chromosome Location:   18:40350329-40355460question
Amplicon Length:   101
Splice Variants Targeted:   ENSRNOT00000000172

Gene Information

enzyme crucial for the metabolism of cysteine [RGD, Feb 2006]

Gene Symbol:   Cdo1
Gene Name:   Cysteine dioxygenase type 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000000158
Entrez:   81718
UniGene:   Rn.2589
Chromosome Mapping:   18q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.210000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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