PrimePCR™ SYBR® Green Assay: Rpl32, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Rpl32, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Rpl32, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0008501
Assay Design:   intron_spanning
Chromosome Location:   4:210999061-211000705question
Amplicon Length:   166
Splice Variants Targeted:   ENSRNOT00000042864 ENSRNOT00000000559 ENSRNOT00000030074 ENSRNOT00000023645 ENSRNOT00000007769 ENSRNOT00000001950 ENSRNOT00000063833 ENSRNOT00000001826 ENSRNOT00000038631 ENSRNOT00000003246 ENSRNOT00000015171 ENSRNOT00000014493 ENSRNOT00000027182 ENSRNOT00000028709 ENSRNOT00000027067 ENSRNOT00000056813 ENSRNOT00000023744 ENSRNOT00000042579 ENSRNOT00000020233 ENSRNOT00000048460 ENSRNOT00000045382

Gene Information

ribosomal protein subunit [RGD, Feb 2006]

Gene Symbol:   Rpl32
Gene Name:   60S ribosomal protein L32
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000010746
Entrez:   28298
Chromosome Mapping:   4q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.200000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download