PrimePCR™ SYBR® Green Assay: Fabp7, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0006118
Assay Design:   intron_spanning
Chromosome Location:   20:42502638-42504605question
Amplicon Length:   98
Splice Variants Targeted:   ENSRNOT00000001079

Gene Information

Brain-type fatty acid-binding protein; may bind docosahexaenoic acid (DHA) as a ligand; may play important role in CNS development [RGD, Feb 2006]

Gene Symbol:   Fabp7
Gene Name:   Fatty acid-binding protein, brain
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000000814
Entrez:   80841
UniGene:   Rn.10014
Chromosome Mapping:   20q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.560000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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