PrimePCR™ SYBR® Green Assay: Sc5dl, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0004199
Assay Design:   intron_spanning
Chromosome Location:   8:45006283-45008340question
Amplicon Length:   116
Splice Variants Targeted:   ENSRNOT00000011284

Gene Information

human homolog is an enzyme involved in catalyzing the conversion of lathosterol into 7-dehydrocholesterol; involved in the synthesis of cholesterol [RGD, Feb 2006]

Gene Symbol:   Sc5dl
Gene Name:   Lathosterol oxidase
Aliases:   Not Available
RefSeq:   NM_053642
Ensembl:   ENSRNOG00000008305
Entrez:   114100
UniGene:   Rn.17462,Rn.18741
Chromosome Mapping:   8q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.990000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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