PrimePCR™ SYBR® Green Assay: Lphn2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Lphn2, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Lphn2, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0001669
Assay Design:   intron_spanning
Chromosome Location:   2:277485871-277487356question
Amplicon Length:   124
Splice Variants Targeted:   ENSRNOT00000003309 ENSRNOT00000045905 ENSRNOT00000042256 ENSRNOT00000040566 ENSRNOT00000045020

Gene Information

low affinity G-protein-coupled receptor for alpha-latrotoxin [RGD, Feb 2006]

Gene Symbol:   Lphn2
Gene Name:   Latrophilin-2 isoform 1 precursor
Aliases:   Not Available
RefSeq:   NM_001190475 NM_134408
Ensembl:   ENSRNOG00000032660
Entrez:   171447
Chromosome Mapping:   2q45

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.820000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download