PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000029988, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: No symbol available for ENSRNOG00000029988, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: No symbol available for ENSRNOG00000029988, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0043907
Assay Design:   exonic
Chromosome Location:   2:42441925-42442049question
Amplicon Length:   95
Splice Variants Targeted:   ENSRNOT00000050073 ENSRNOT00000051060 ENSRNOT00000042581 ENSRNOT00000066508 ENSRNOT00000035009 ENSRNOT00000036223 ENSRNOT00000043704 ENSRNOT00000049292 ENSRNOT00000002324 ENSRNOT00000016340 ENSRNOT00000026333 ENSRNOT00000061219 ENSRNOT00000041777 ENSRNOT00000040029 ENSRNOT00000067688 ENSRNOT00000015034 ENSRNOT00000014939 ENSRNOT00000042983 ENSRNOT00000003180 ENSRNOT00000045579 ENSRNOT00000047012 ENSRNOT00000046772 ENSRNOT00000043110 ENSRNOT00000045531

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000029988
Gene Name:   Protein LOC100361079
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000029988
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.750000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download