PrimePCR™ Probe Assay: Olr1688, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Olr1688, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Olr1688, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0041633
Assay Design:   exonic
Chromosome Location:   20:719433-719522question
Amplicon Length:   60
Splice Variants Targeted:   ENSRNOT00000075301 ENSRNOT00000074751 ENSRNOT00000047855 ENSRNOT00000010865 ENSRNOT00000073543 ENSRNOT00000072457 ENSRNOT00000049250 ENSRNOT00000052186 ENSRNOT00000040957 ENSRNOT00000074181 ENSRNOT00000072222 ENSRNOT00000073732 ENSRNOT00000071933 ENSRNOT00000075267 ENSRNOT00000074388 ENSRNOT00000072544 ENSRNOT00000075252 ENSRNOT00000052052 ENSRNOT00000041189 ENSRNOT00000044130 ENSRNOT00000045794 ENSRNOT00000024937 ENSRNOT00000067342 ENSRNOT00000052199 ENSRNOT00000049595

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr1688
Gene Name:   Olfactory receptor Olr1688
Aliases:   Not Available
RefSeq:   NM_001000275
Ensembl:   ENSRNOG00000050612
Entrez:   294164
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.250000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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