PrimePCR™ Probe Assay: Ces2c, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0040014
Assay Design:   exonic
Chromosome Location:   1:289976778-289977777question
Amplicon Length:   71
Splice Variants Targeted:   ENSRNOT00000047530 ENSRNOT00000073241 ENSRNOT00000074303

Gene Information

enzyme that is responsible for the hydrolysis of long-chain fatty acids and thioesters; may play a role in lipid metabolism and/or the detoxification of certain drugs [RGD, Feb 2006]

Gene Symbol:   Ces2c
Gene Name:   Protein Ces2c; RCG57590
Aliases:   Not Available
RefSeq:   NM_133586
Ensembl:   ENSRNOG00000047661
Entrez:   171118
UniGene:   Rn.14535
Chromosome Mapping:   1q55

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.980000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download