PrimePCR™ Probe Assay: Rps20, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Rps20, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Rps20, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0038550
Assay Design:   exonic
Chromosome Location:   3:28188789-28188881question
Amplicon Length:   63
Splice Variants Targeted:   ENSRNOT00000011314 ENSRNOT00000072841 ENSRNOT00000031928 ENSRNOT00000033234 ENSRNOT00000073274 ENSRNOT00000015944 ENSRNOT00000045982 ENSRNOT00000040481 ENSRNOT00000047587 ENSRNOT00000041813

Gene Information

40 S ribosomal subunit protein [RGD, Feb 2006]

Gene Symbol:   Rps20
Gene Name:   40S ribosomal protein S20
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000029627
Entrez:   122772
Chromosome Mapping:   5q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.210000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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