PrimePCR™ Probe Assay: Olr1469, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Olr1469, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Olr1469, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0038209
Assay Design:   exonic
Chromosome Location:   10:59968333-59968469question
Amplicon Length:   107
Splice Variants Targeted:   ENSRNOT00000009566 ENSRNOT00000026857 ENSRNOT00000072913 ENSRNOT00000052183 ENSRNOT00000065541 ENSRNOT00000074380 ENSRNOT00000045573 ENSRNOT00000073603 ENSRNOT00000041318 ENSRNOT00000005428 ENSRNOT00000042409 ENSRNOT00000041835 ENSRNOT00000045221 ENSRNOT00000074729 ENSRNOT00000074648 ENSRNOT00000048492 ENSRNOT00000071349 ENSRNOT00000071144 ENSRNOT00000049537 ENSRNOT00000041085 ENSRNOT00000050847 ENSRNOT00000066983 ENSRNOT00000072702 ENSRNOT00000070956

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr1469
Gene Name:   Olfactory receptor 1
Aliases:   Not Available
RefSeq:   NM_212527
Ensembl:   ENSRNOG00000049973
Entrez:   404976
Chromosome Mapping:   10q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.810000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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