PrimePCR™ Probe Assay: Olr158, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Olr158, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Olr158, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0037620
Assay Design:   exonic
Chromosome Location:   1:176630953-176631095question
Amplicon Length:   113
Splice Variants Targeted:   ENSRNOT00000051371 ENSRNOT00000040759 ENSRNOT00000048459 ENSRNOT00000049531 ENSRNOT00000043180 ENSRNOT00000045459 ENSRNOT00000017975 ENSRNOT00000047737 ENSRNOT00000019979 ENSRNOT00000001432 ENSRNOT00000001807 ENSRNOT00000047609 ENSRNOT00000016042 ENSRNOT00000051265 ENSRNOT00000018004 ENSRNOT00000052017 ENSRNOT00000041571 ENSRNOT00000009268 ENSRNOT00000051846 ENSRNOT00000067542 ENSRNOT00000002879 ENSRNOT00000048754 ENSRNOT00000023315 ENSRNOT00000025773 ENSRNOT00000071514 ENSRNOT00000074466 ENSRNO

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr158
Gene Name:   Olfactory receptor Olr158
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049474
Entrez:   365334
Chromosome Mapping:   1q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.750000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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