PrimePCR™ Probe Assay: Hus1b, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Hus1b, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Hus1b, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0036186
Assay Design:   exonic
Chromosome Location:   17:36739067-36739181question
Amplicon Length:   85
Splice Variants Targeted:   ENSRNOT00000039177 ENSRNOT00000016797 ENSRNOT00000040373 ENSRNOT00000022534 ENSRNOT00000047960 ENSRNOT00000045913 ENSRNOT00000043854 ENSRNOT00000074097 ENSRNOT00000072505 ENSRNOT00000009898

Gene Information

Description Not Available

Gene Symbol:   Hus1b
Gene Name:   Checkpoint protein HUS1B
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049323
Entrez:   691382
Chromosome Mapping:   17p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.960000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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