PrimePCR™ Probe Assay: Vom2r67, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Vom2r67, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Vom2r67, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0035880
Assay Design:   exonic
Chromosome Location:   14:1315285-1315378question
Amplicon Length:   64
Splice Variants Targeted:   ENSRNOT00000071844 ENSRNOT00000073174 ENSRNOT00000040119 ENSRNOT00000073222 ENSRNOT00000074792 ENSRNOT00000074699 ENSRNOT00000042693 ENSRNOT00000073754 ENSRNOT00000062058 ENSRNOT00000050916 ENSRNOT00000034630 ENSRNOT00000044505 ENSRNOT00000075323 ENSRNOT00000072621 ENSRNOT00000074130 ENSRNOT00000074598 ENSRNOT00000048946 ENSRNOT00000073032 ENSRNOT00000072232 ENSRNOT00000048588 ENSRNOT00000050241 ENSRNOT00000033032 ENSRNOT00000050133 ENSRNOT00000049544 ENSRNOT00000061889 ENSRNOT00000073861 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   Vom2r67
Gene Name:   Vomeronasal 2 receptor, 67
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000031928
Entrez:   689485
Chromosome Mapping:   14p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.480000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download