PrimePCR™ Probe Assay: 3110082J24Rik, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: 3110082J24Rik, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: 3110082J24Rik, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0034551
Assay Design:   exonic
Chromosome Location:   6:37453806-37453907question
Amplicon Length:   72
Splice Variants Targeted:   ENSRNOT00000041025 ENSRNOT00000043971 ENSRNOT00000045699 ENSRNOT00000001290 ENSRNOT00000040393 ENSRNOT00000016020 ENSRNOT00000024704 ENSRNOT00000064347 ENSRNOT00000004408 ENSRNOT00000058274 ENSRNOT00000029255 ENSRNOT00000058276 ENSRNOT00000002316 ENSRNOT00000001235 ENSRNOT00000064551 ENSRNOT00000052063 ENSRNOT00000059496 ENSRNOT00000045515 ENSRNOT00000010683 ENSRNOT00000035338 ENSRNOT00000011906 ENSRNOT00000048770 ENSRNOT00000042642 ENSRNOT00000040762 ENSRNOT00000006311 ENSRNOT00000016492 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   3110082J24Rik
Gene Name:   RIKEN cDNA 3110082J24 gene
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046262
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.850000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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