PrimePCR™ Probe Assay: TMSB10, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0033974
Assay Design:   exonic
Chromosome Location:   7:122416570-122416676question
Amplicon Length:   77
Splice Variants Targeted:   ENSRNOT00000075393 ENSRNOT00000023430 ENSRNOT00000008480 ENSRNOT00000059524 ENSRNOT00000029435 ENSRNOT00000033886 ENSRNOT00000022846 ENSRNOT00000041880 ENSRNOT00000028553 ENSRNOT00000068233 ENSRNOT00000003087 ENSRNOT00000074927 ENSRNOT00000054897 ENSRNOT00000055447 ENSRNOT00000057740

Gene Information

Description Not Available

Gene Symbol:   TMSB10
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000036921
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 37.290000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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