PrimePCR™ Probe Assay: Hist1h2bc, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Hist1h2bc, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Hist1h2bc, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0033012
Assay Design:   exonic
Chromosome Location:   17:58943026-58943137question
Amplicon Length:   82
Splice Variants Targeted:   ENSRNOT00000041025 ENSRNOT00000043971 ENSRNOT00000045699 ENSRNOT00000038203 ENSRNOT00000017712 ENSRNOT00000008328 ENSRNOT00000004329 ENSRNOT00000022522 ENSRNOT00000046129 ENSRNOT00000072269 ENSRNOT00000022630 ENSRNOT00000072195 ENSRNOT00000030198 ENSRNOT00000075811 ENSRNOT00000072293 ENSRNOT00000071688 ENSRNOT00000041423 ENSRNOT00000048465 ENSRNOT00000049001

Gene Information

Description Not Available

Gene Symbol:   Hist1h2bc
Gene Name:   Histone H2B
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000047183
Entrez:   680312
Chromosome Mapping:   17q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.980000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download