PrimePCR™ Probe Assay: Cx3cr1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0028471
Assay Design:   exonic
Chromosome Location:   8:127941880-127942023question
Amplicon Length:   114
Splice Variants Targeted:   ENSRNOT00000025019

Gene Information

member of the rhodopsin family of G-protein coupled receptors; may act as a receptor for a chemokine peptide ligand [RGD, Feb 2006]

Gene Symbol:   Cx3cr1
Gene Name:   CX3C chemokine receptor 1
Aliases:   Not Available
RefSeq:   NM_133534
Ensembl:   ENSRNOG00000018509
Entrez:   171056
UniGene:   Rn.10482
Chromosome Mapping:   8q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.880000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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