PrimePCR™ Probe Assay: Tap1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0028442
Assay Design:   exonic
Chromosome Location:   20:6061521-6061653question
Amplicon Length:   103
Splice Variants Targeted:   ENSRNOT00000000529

Gene Information

has similarity to multidrug resistant transporters; may transport antigenic peptides across the endoplasmic reticulum membrane in preparation for MHC class I presentation [RGD, Feb 2006]

Gene Symbol:   Tap1
Gene Name:   Antigen peptide transporter 1 precursor
Aliases:   Not Available
RefSeq:   NM_032055
Ensembl:   ENSRNOG00000000457
Entrez:   24811
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.510000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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