PrimePCR™ Probe Assay: Gp5, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0028027
Assay Design:   exonic
Chromosome Location:   11:77079148-77079280question
Amplicon Length:   103
Splice Variants Targeted:   ENSRNOT00000040884

Gene Information

major surface protein cleaved during thrombin-induced platelet activation; may bind GPIb-IX complex to form GPIb-V-IX, a receptor for von Willebrand factor and thrombin [RGD, Feb 2006]

Gene Symbol:   Gp5
Gene Name:   Platelet glycoprotein V precursor
Aliases:   Not Available
RefSeq:   NM_012795
Ensembl:   ENSRNOG00000038540
Entrez:   25259
UniGene:   Rn.138111
Chromosome Mapping:   11q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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