PrimePCR™ Probe Assay: Opa1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0027800
Assay Design:   exonic
Chromosome Location:   11:77811387-77812079question
Amplicon Length:   76
Splice Variants Targeted:   ENSRNOT00000063999 ENSRNOT00000002338 ENSRNOT00000002343 ENSRNOT00000045766 ENSRNOT00000043627

Gene Information

Description Not Available

Gene Symbol:   Opa1
Gene Name:   Dynamin-like 120 kDa protein, mitochondrial Dynamin-like 120 kDa protein, form S1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000001717
Entrez:   171116
Chromosome Mapping:   11q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.100000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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