PrimePCR™ Probe Assay: Ptger1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0026376
Assay Design:   exonic
Chromosome Location:   19:35779929-35780020question
Amplicon Length:   62
Splice Variants Targeted:   ENSRNOT00000005470

Gene Information

binds prostaglandin E2; plays a role in G-protein coupled receptor signaling and Ca2+ mobilization [RGD, Feb 2006]

Gene Symbol:   Ptger1
Gene Name:   Prostaglandin E2 receptor EP1 subtype
Aliases:   Not Available
RefSeq:   NM_013100
Ensembl:   ENSRNOG00000004094
Entrez:   25637
Chromosome Mapping:   19q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.570000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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