PrimePCR™ Probe Assay: Ahsg, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0025739
Assay Design:   exonic
Chromosome Location:   11:84802883-84803338question
Amplicon Length:   96
Splice Variants Targeted:   ENSRNOT00000058422

Gene Information

inhibits insulin receptor tyrosine kinase activity; may be involved in bone mineralization and immune response [RGD, Feb 2006]

Gene Symbol:   Ahsg
Gene Name:   Alpha-2-HS-glycoprotein precursor
Aliases:   Not Available
RefSeq:   NM_012898
Ensembl:   ENSRNOG00000038370
Entrez:   25373
UniGene:   Rn.32083
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.994200
y-intercept 38.150000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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