PrimePCR™ Probe Assay: Rhob, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0025125
Assay Design:   exonic
Chromosome Location:   6:43474303-43474404question
Amplicon Length:   72
Splice Variants Targeted:   ENSRNOT00000008008

Gene Information

member of the ras gene superfamily; GTP-binding proteins [RGD, Feb 2006]

Gene Symbol:   Rhob
Gene Name:   Rho-related GTP-binding protein RhoB
Aliases:   Not Available
RefSeq:   NM_022542
Ensembl:   ENSRNOG00000021403
Entrez:   64373
UniGene:   Rn.2042
Chromosome Mapping:   6q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.660000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download