PrimePCR™ Probe Assay: Tuba3b, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0023988
Assay Design:   exonic
Chromosome Location:   4:244085332-244085473question
Amplicon Length:   112
Splice Variants Targeted:   ENSRNOT00000052352

Gene Information

Description Not Available

Gene Symbol:   Tuba3b
Gene Name:   Tubulin alpha-3 chain
Aliases:   Not Available
RefSeq:   NM_001024336
Ensembl:   ENSRNOG00000032967
Entrez:   500363
UniGene:   Rn.123187
Chromosome Mapping:   4q44

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 36.450000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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