PrimePCR™ Probe Assay: Slc32a1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $208.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0023792
Assay Design:   exonic
Chromosome Location:   3:160501981-160502074question
Amplicon Length:   64
Splice Variants Targeted:   ENSRNOT00000020720

Gene Information

integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles [RGD, Feb 2006]

Gene Symbol:   Slc32a1
Gene Name:   Vesicular inhibitory amino acid transporter
Aliases:   Not Available
RefSeq:   NM_031782
Ensembl:   ENSRNOG00000015393
Entrez:   83612
UniGene:   Rn.10846
Chromosome Mapping:   3q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.210000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download