PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000050487, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000050487, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000050487, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0056917
Assay Design:   Exonic
Chromosome Location:   15:36164945-36165061question
Amplicon Length:   87
Splice Variants Targeted:   ENSRNOT00000072362 ENSRNOT00000071061 ENSRNOT00000075194 ENSRNOT00000073984 ENSRNOT00000071631 ENSRNOT00000071740 ENSRNOT00000073175 ENSRNOT00000075456 ENSRNOT00000064158 ENSRNOT00000072382 ENSRNOT00000071598 ENSRNOT00000073855 ENSRNOT00000067135 ENSRNOT00000025674 ENSRNOT00000007640 ENSRNOT00000070873 ENSRNOT00000006862 ENSRNOT00000010043 ENSRNOT00000021030 ENSRNOT00000036808 ENSRNOT00000036848 ENSRNOT00000022236 ENSRNOT00000026586 ENSRNOT00000028665

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000050487
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050487
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 37.560000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download