PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000050972, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000050972, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000050972, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0055229
Assay Design:   Exonic
Chromosome Location:   6:147673316-147673418question
Amplicon Length:   73
Splice Variants Targeted:   ENSRNOT00000001551 ENSRNOT00000001552 ENSRNOT00000039281 ENSRNOT00000009083 ENSRNOT00000065383 ENSRNOT00000057058 ENSRNOT00000071078 ENSRNOT00000075284 ENSRNOT00000071945 ENSRNOT00000070980 ENSRNOT00000075376 ENSRNOT00000073207 ENSRNOT00000072566 ENSRNOT00000044682 ENSRNOT00000072990 ENSRNOT00000072143 ENSRNOT00000074540 ENSRNOT00000068284 ENSRNOT00000070810 ENSRNOT00000074565 ENSRNOT00000058552 ENSRNOT00000048790 ENSRNOT00000057122 ENSRNOT00000057120 ENSRNOT00000075502

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000050972
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050972
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 37.510000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download