PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000050730, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000050730, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000050730, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0055201
Assay Design:   Exonic
Chromosome Location:   3:158029498-158029642question
Amplicon Length:   115
Splice Variants Targeted:   ENSRNOT00000039927 ENSRNOT00000036706 ENSRNOT00000045607 ENSRNOT00000061340 ENSRNOT00000032824 ENSRNOT00000043945 ENSRNOT00000039569 ENSRNOT00000035699 ENSRNOT00000032908 ENSRNOT00000003755 ENSRNOT00000008094 ENSRNOT00000035594 ENSRNOT00000000455 ENSRNOT00000040427 ENSRNOT00000039932 ENSRNOT00000047410 ENSRNOT00000043042 ENSRNOT00000036106 ENSRNOT00000046064 ENSRNOT00000041811 ENSRNOT00000013168 ENSRNOT00000008851 ENSRNOT00000045544 ENSRNOT00000047868 ENSRNOT00000047948 ENSRNOT00000011090 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000050730
Gene Name:   Glyceraldehyde-3-phosphate dehydrogenase
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050730
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 37.500000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download