PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000049741, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000049741, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000049741, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0055088
Assay Design:   Exonic
Chromosome Location:   18:17277036-17342606question
Amplicon Length:   152
Splice Variants Targeted:   ENSRNOT00000046326 ENSRNOT00000071026 ENSRNOT00000074210 ENSRNOT00000071111 ENSRNOT00000072808 ENSRNOT00000072844 ENSRNOT00000044462 ENSRNOT00000074318 ENSRNOT00000072017 ENSRNOT00000045865 ENSRNOT00000073463 ENSRNOT00000071759 ENSRNOT00000074243 ENSRNOT00000075319 ENSRNOT00000073843 ENSRNOT00000071051 ENSRNOT00000074798 ENSRNOT00000073434 ENSRNOT00000071621 ENSRNOT00000073584 ENSRNOT00000073727 ENSRNOT00000049046 ENSRNOT00000072956 ENSRNOT00000075255 ENSRNOT00000042249 ENSRNOT00000075481 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000049741
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049741
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.510000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download