PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000032289, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000032289, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000032289, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0054397
Assay Design:   Exonic
Chromosome Location:   9:65855031-65855207question
Amplicon Length:   147
Splice Variants Targeted:   ENSRNOT00000061344 ENSRNOT00000038635 ENSRNOT00000043480 ENSRNOT00000023108 ENSRNOT00000052018 ENSRNOT00000017349 ENSRNOT00000048738 ENSRNOT00000071178 ENSRNOT00000002656 ENSRNOT00000065882 ENSRNOT00000004583 ENSRNOT00000019508 ENSRNOT00000072013 ENSRNOT00000000365 ENSRNOT00000036337 ENSRNOT00000058491 ENSRNOT00000007876 ENSRNOT00000003897 ENSRNOT00000042870 ENSRNOT00000044776 ENSRNOT00000056041 ENSRNOT00000021937 ENSRNOT00000047960 ENSRNOT00000045913 ENSRNOT00000011551 ENSRNOT00000010437 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000032289
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000032289
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 38.280000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download