PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000025046, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000025046, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000025046, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0054225
Assay Design:   Exonic
Chromosome Location:   20:32691379-32691515question
Amplicon Length:   107
Splice Variants Targeted:   ENSRNOT00000064632 ENSRNOT00000023364 ENSRNOT00000045124 ENSRNOT00000032406 ENSRNOT00000061340 ENSRNOT00000012714 ENSRNOT00000008094 ENSRNOT00000035216 ENSRNOT00000040427 ENSRNOT00000039932 ENSRNOT00000044035 ENSRNOT00000034533 ENSRNOT00000037429 ENSRNOT00000047410 ENSRNOT00000036106 ENSRNOT00000041811 ENSRNOT00000049603 ENSRNOT00000047137 ENSRNOT00000023343 ENSRNOT00000050443 ENSRNOT00000026712 ENSRNOT00000051417 ENSRNOT00000044998 ENSRNOT00000042076 ENSRNOT00000033178 ENSRNOT00000045126 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000025046
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000025046
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 37.300000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download