PrimePCR™ SYBR® Green Assay: Mcpt8l3, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Mcpt8l3, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Mcpt8l3, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0053033
Assay Design:   Exonic
Chromosome Location:   15:38557581-38558192question
Amplicon Length:   172
Splice Variants Targeted:   ENSRNOT00000020697 ENSRNOT00000049315 ENSRNOT00000012038 ENSRNOT00000057902 ENSRNOT00000004692 ENSRNOT00000073225 ENSRNOT00000065706 ENSRNOT00000038306 ENSRNOT00000038285 ENSRNOT00000011292 ENSRNOT00000033194 ENSRNOT00000035125

Gene Information

predicted to act as a serine protease [RGD, Feb 2006]

Gene Symbol:   Mcpt8l3
Gene Name:   Protein Mcpt8l3
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050368
Entrez:   408207
Chromosome Mapping:   15p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.994800
y-intercept 38.800000
Efficiency 87

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download