PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000025442, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000025442, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000025442, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0022400
Assay Design:   exonic
Chromosome Location:   X:105531555-105531671question
Amplicon Length:   85
Splice Variants Targeted:   ENSRNOT00000011793 ENSRNOT00000034681 ENSRNOT00000039250 ENSRNOT00000032631 ENSRNOT00000032995 ENSRNOT00000033201 ENSRNOT00000038120 ENSRNOT00000011239 ENSRNOT00000037571 ENSRNOT00000037416 ENSRNOT00000014849 ENSRNOT00000051929 ENSRNOT00000048888 ENSRNOT00000057412 ENSRNOT00000042570 ENSRNOT00000047001 ENSRNOT00000050331 ENSRNOT00000043027 ENSRNOT00000049689

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000025442
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000025442
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.090000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download