PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000032912, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $138.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0022360
Assay Design:   exonic
Chromosome Location:   8:92979927-92980057question
Amplicon Length:   101
Splice Variants Targeted:   ENSRNOT00000033185 ENSRNOT00000009431 ENSRNOT00000040358 ENSRNOT00000044750 ENSRNOT00000046844 ENSRNOT00000041458 ENSRNOT00000073061 ENSRNOT00000075369 ENSRNOT00000021846 ENSRNOT00000052249 ENSRNOT00000009702

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000032912
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000032912
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.800000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download