PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000046638, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000046638, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000046638, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0022349
Assay Design:   exonic
Chromosome Location:   X:100466840-100467157question
Amplicon Length:   88
Splice Variants Targeted:   ENSRNOT00000035992 ENSRNOT00000072151 ENSRNOT00000072930 ENSRNOT00000021742 ENSRNOT00000030857 ENSRNOT00000033772 ENSRNOT00000042498 ENSRNOT00000047433 ENSRNOT00000043099 ENSRNOT00000031481 ENSRNOT00000070851 ENSRNOT00000006697 ENSRNOT00000075740 ENSRNOT00000042638 ENSRNOT00000066520 ENSRNOT00000073235 ENSRNOT00000061526 ENSRNOT00000073416 ENSRNOT00000008101 ENSRNOT00000008047 ENSRNOT00000075257 ENSRNOT00000075429 ENSRNOT00000042110 ENSRNOT00000063989 ENSRNOT00000075012 ENSRNOT00000072043 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000046638
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046638
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.120000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download