PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000032435, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000032435, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000032435, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0022275
Assay Design:   exonic
Chromosome Location:   2:177053888-177054062question
Amplicon Length:   127
Splice Variants Targeted:   ENSRNOT00000045926 ENSRNOT00000034749 ENSRNOT00000012714 ENSRNOT00000033359 ENSRNOT00000008094 ENSRNOT00000065264 ENSRNOT00000000455 ENSRNOT00000039932 ENSRNOT00000040732 ENSRNOT00000013168 ENSRNOT00000026387 ENSRNOT00000025879 ENSRNOT00000043243 ENSRNOT00000047137 ENSRNOT00000035280 ENSRNOT00000030148 ENSRNOT00000050443 ENSRNOT00000055125 ENSRNOT00000011894 ENSRNOT00000002985 ENSRNOT00000034563 ENSRNOT00000047750 ENSRNOT00000058917 ENSRNOT00000041328 ENSRNOT00000043709 ENSRNOT00000047820

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000032435
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000032435
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 36.640000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download