PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000009383, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000009383, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000009383, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0022213
Assay Design:   exonic
Chromosome Location:   2:211910084-211910229question
Amplicon Length:   116
Splice Variants Targeted:   ENSRNOT00000060470 ENSRNOT00000050092 ENSRNOT00000033073 ENSRNOT00000001905 ENSRNOT00000018935 ENSRNOT00000064526 ENSRNOT00000044246 ENSRNOT00000025948 ENSRNOT00000000676 ENSRNOT00000013474 ENSRNOT00000073277 ENSRNOT00000001866 ENSRNOT00000065477 ENSRNOT00000008535 ENSRNOT00000065533 ENSRNOT00000034961 ENSRNOT00000013193 ENSRNOT00000018454 ENSRNOT00000017294 ENSRNOT00000065884 ENSRNOT00000075788 ENSRNOT00000072995 ENSRNOT00000065242 ENSRNOT00000056485 ENSRNOT00000045938 ENSRNOT00000012420 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000009383
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000009383
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.710000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download