PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000028955, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000028955, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000028955, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0020724
Assay Design:   exonic
Chromosome Location:   1:222040347-222040626question
Amplicon Length:   155
Splice Variants Targeted:   ENSRNOT00000073239 ENSRNOT00000008759 ENSRNOT00000049771 ENSRNOT00000068171 ENSRNOT00000025927 ENSRNOT00000017730 ENSRNOT00000028786 ENSRNOT00000022658 ENSRNOT00000015638 ENSRNOT00000014441 ENSRNOT00000014211 ENSRNOT00000023062 ENSRNOT00000043872 ENSRNOT00000037313 ENSRNOT00000074195 ENSRNOT00000045964 ENSRNOT00000059447 ENSRNOT00000005583 ENSRNOT00000014505 ENSRNOT00000043082 ENSRNOT00000011049 ENSRNOT00000052087 ENSRNOT00000066122 ENSRNOT00000007768 ENSRNOT00000044234 ENSRNOT00000033863 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000028955
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000028955
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.550000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download