PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000043400, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000043400, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000043400, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0020602
Assay Design:   exonic
Chromosome Location:   4:143704538-143704644question
Amplicon Length:   77
Splice Variants Targeted:   ENSRNOT00000022134 ENSRNOT00000049180 ENSRNOT00000024186 ENSRNOT00000007977 ENSRNOT00000006849 ENSRNOT00000018246 ENSRNOT00000068441 ENSRNOT00000007941 ENSRNOT00000004147 ENSRNOT00000057001 ENSRNOT00000022951 ENSRNOT00000075819 ENSRNOT00000068775 ENSRNOT00000007133 ENSRNOT00000065501 ENSRNOT00000061999 ENSRNOT00000021905 ENSRNOT00000031172 ENSRNOT00000025218 ENSRNOT00000032477 ENSRNOT00000065897 ENSRNOT00000014022

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000043400
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000043400
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download