PrimePCR™ SYBR® Green Assay: Vom1r-ps39, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Vom1r-ps39, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Vom1r-ps39, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0019377
Assay Design:   exonic
Chromosome Location:   1:69027123-69027236question
Amplicon Length:   84
Splice Variants Targeted:   ENSRNOT00000014795 ENSRNOT00000047482 ENSRNOT00000068663 ENSRNOT00000061224 ENSRNOT00000035908 ENSRNOT00000044950 ENSRNOT00000022734 ENSRNOT00000070823 ENSRNOT00000043828 ENSRNOT00000051034 ENSRNOT00000074949

Gene Information

Description Not Available

Gene Symbol:   Vom1r-ps39
Gene Name:   Vomeronasal 1 receptor pseudogene 39
Aliases:   Not Available
RefSeq:   NM_001009509
Ensembl:   ENSRNOG00000047242
Entrez:   494228
Chromosome Mapping:   1q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.994800
y-intercept 35.980000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download