PrimePCR™ SYBR® Green Assay: SPSB2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SPSB2, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SPSB2, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0017318
Assay Design:   exonic
Chromosome Location:   9:19094007-19094145question
Amplicon Length:   109
Splice Variants Targeted:   ENSRNOT00000027372 ENSRNOT00000027393 ENSRNOT00000027343 ENSRNOT00000027340 ENSRNOT00000006345 ENSRNOT00000044395 ENSRNOT00000030519 ENSRNOT00000072086 ENSRNOT00000002530 ENSRNOT00000009067 ENSRNOT00000010634 ENSRNOT00000073209 ENSRNOT00000020493 ENSRNOT00000008019 ENSRNOT00000065811 ENSRNOT00000025388 ENSRNOT00000016289 ENSRNOT00000026186 ENSRNOT00000075598

Gene Information

Description Not Available

Gene Symbol:   SPSB2
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000047113
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.990700
y-intercept 39.630000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download