PrimePCR™ SYBR® Green Assay: Kcnip2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Kcnip2, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Kcnip2, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0016833
Assay Design:   exonic
Chromosome Location:   1:272981248-272981740question
Amplicon Length:   63
Splice Variants Targeted:   ENSRNOT00000068278 ENSRNOT00000024709 ENSRNOT00000024750 ENSRNOT00000055915 ENSRNOT00000048592 ENSRNOT00000044195

Gene Information

Ca(2+)-binding protein that acts as an auxiliary subunit for voltage-gated K(+) channels [RGD, Feb 2006]

Gene Symbol:   Kcnip2
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   NM_020094 NM_020095 NM_001033961
Ensembl:   ENSRNOG00000050450
Entrez:   56817
Chromosome Mapping:   1q54

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.850000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download