PrimePCR™ SYBR® Green Assay: RGD1564688, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RGD1564688, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGD1564688, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0016567
Assay Design:   exonic
Chromosome Location:   11:75185030-75185179question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000021921 ENSRNOT00000061340 ENSRNOT00000035699 ENSRNOT00000008094 ENSRNOT00000050443 ENSRNOT00000047750 ENSRNOT00000058917 ENSRNOT00000041328 ENSRNOT00000043709 ENSRNOT00000047820

Gene Information

Description Not Available

Gene Symbol:   RGD1564688
Gene Name:   Glyceraldehyde-3-phosphate dehydrogenase
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000038610
Entrez:   498099
Chromosome Mapping:   11q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.280000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download