PrimePCR™ SYBR® Green Assay: RGD1562265, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RGD1562265, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGD1562265, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0015939
Assay Design:   exonic
Chromosome Location:   5:48896397-48896503question
Amplicon Length:   77
Splice Variants Targeted:   ENSRNOT00000027225 ENSRNOT00000004416 ENSRNOT00000006370 ENSRNOT00000005078 ENSRNOT00000056366 ENSRNOT00000015578 ENSRNOT00000068663 ENSRNOT00000023079 ENSRNOT00000015386 ENSRNOT00000037255 ENSRNOT00000055615 ENSRNOT00000064548 ENSRNOT00000022184 ENSRNOT00000028644 ENSRNOT00000075181 ENSRNOT00000067777 ENSRNOT00000042939 ENSRNOT00000075079 ENSRNOT00000049732

Gene Information

Description Not Available

Gene Symbol:   RGD1562265
Gene Name:   40S ribosomal protein S12
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000032454
Entrez:   313283
Chromosome Mapping:   5q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 35.970000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download