PrimePCR™ SYBR® Green Assay: 9430038I01Rik, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: 9430038I01Rik, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: 9430038I01Rik, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0015214
Assay Design:   exonic
Chromosome Location:   1:216647023-216647150question
Amplicon Length:   98
Splice Variants Targeted:   ENSRNOT00000006345 ENSRNOT00000042006 ENSRNOT00000037685 ENSRNOT00000047314 ENSRNOT00000027932 ENSRNOT00000057845 ENSRNOT00000045056 ENSRNOT00000016912 ENSRNOT00000014158 ENSRNOT00000019754 ENSRNOT00000067663 ENSRNOT00000039341 ENSRNOT00000036647 ENSRNOT00000024932 ENSRNOT00000064841 ENSRNOT00000017213 ENSRNOT00000068275 ENSRNOT00000074734 ENSRNOT00000028322 ENSRNOT00000068434 ENSRNOT00000073093 ENSRNOT00000028697 ENSRNOT00000018584

Gene Information

Description Not Available

Gene Symbol:   9430038I01Rik
Gene Name:   RIKEN cDNA 9430038I01 gene
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050554
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.990000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download