PrimePCR™ SYBR® Green Assay: Hes2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0014718
Assay Design:   exonic
Chromosome Location:   5:172893218-172893390question
Amplicon Length:   63
Splice Variants Targeted:   ENSRNOT00000014035

Gene Information

helix-loop-helix transcription factor; may act as a negative regulator of gene transcription [RGD, Feb 2006]

Gene Symbol:   Hes2
Gene Name:   Transcription factor HES-2
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000010490
Entrez:   29567
UniGene:   Rn.44655
Chromosome Mapping:   5q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.620000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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