PrimePCR™ SYBR® Green Assay: Olr1257, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Olr1257, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Olr1257, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0012920
Assay Design:   exonic
Chromosome Location:   4:1811239-1811351question
Amplicon Length:   83
Splice Variants Targeted:   ENSRNOT00000073106 ENSRNOT00000045736 ENSRNOT00000073532 ENSRNOT00000073166 ENSRNOT00000074529 ENSRNOT00000042784 ENSRNOT00000043680 ENSRNOT00000045293 ENSRNOT00000075071 ENSRNOT00000074552 ENSRNOT00000045171 ENSRNOT00000048785 ENSRNOT00000074038 ENSRNOT00000073505 ENSRNOT00000043212 ENSRNOT00000047744 ENSRNOT00000026488 ENSRNOT00000042829 ENSRNOT00000073807 ENSRNOT00000049347 ENSRNOT00000071070 ENSRNOT00000072782 ENSRNOT00000075043 ENSRNOT00000075478 ENSRNOT00000073972 ENSRNOT00000075645 ENSRNO

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr1257
Gene Name:   Olfactory receptor Olr1257
Aliases:   Not Available
RefSeq:   NM_001000596
Ensembl:   ENSRNOG00000048360
Entrez:   367061
Chromosome Mapping:   8q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 36.480000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download