PrimePCR™ SYBR® Green Assay: RGD1562725, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: RGD1562725, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGD1562725, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0012127
Assay Design:   exonic
Chromosome Location:   5:155753020-155753150question
Amplicon Length:   101
Splice Variants Targeted:   ENSRNOT00000011314 ENSRNOT00000059565 ENSRNOT00000008160 ENSRNOT00000032311 ENSRNOT00000045982 ENSRNOT00000040481 ENSRNOT00000047587 ENSRNOT00000042264 ENSRNOT00000041813 ENSRNOT00000048346 ENSRNOT00000043008 ENSRNOT00000050041 ENSRNOT00000072213

Gene Information

Description Not Available

Gene Symbol:   RGD1562725
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000033541
Entrez:   500559
Chromosome Mapping:   5q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.640000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download