PrimePCR™ SYBR® Green Assay: Krtap3-3l1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Krtap3-3l1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Krtap3-3l1, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0012011
Assay Design:   exonic
Chromosome Location:   10:87271665-87271796question
Amplicon Length:   102
Splice Variants Targeted:   ENSRNOT00000038449 ENSRNOT00000002692 ENSRNOT00000044378 ENSRNOT00000023140 ENSRNOT00000063882 ENSRNOT00000059602 ENSRNOT00000029041 ENSRNOT00000014791 ENSRNOT00000027829 ENSRNOT00000009825 ENSRNOT00000073863 ENSRNOT00000064083 ENSRNOT00000028008 ENSRNOT00000068156 ENSRNOT00000024679 ENSRNOT00000031796 ENSRNOT00000049895 ENSRNOT00000032248 ENSRNOT00000005903 ENSRNOT00000052087 ENSRNOT00000014129 ENSRNOT00000055822 ENSRNOT00000065288 ENSRNOT00000039983 ENSRNOT00000045189

Gene Information

Description Not Available

Gene Symbol:   Krtap3-3l1
Gene Name:   Protein Krtap3-3; Protein Krtap3-3l1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049383
Entrez:   680060
Chromosome Mapping:   10q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 37.010000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download