PrimePCR™ SYBR® Green Assay: Mpa2l, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Mpa2l, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Mpa2l, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0011706
Assay Design:   exonic
Chromosome Location:   14:5923603-5923726question
Amplicon Length:   94
Splice Variants Targeted:   ENSRNOT00000017556 ENSRNOT00000057227 ENSRNOT00000014572 ENSRNOT00000045678 ENSRNOT00000042726 ENSRNOT00000046315 ENSRNOT00000047854 ENSRNOT00000045314 ENSRNOT00000055160 ENSRNOT00000071877 ENSRNOT00000040985 ENSRNOT00000074889

Gene Information

Description Not Available

Gene Symbol:   Mpa2l
Gene Name:   Protein Mpa2l
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000002134
Entrez:   305139
Chromosome Mapping:   14p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.790000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download